International Journal of Advance Agricultural Research
ISSN: 2053-1265
Vol. 6(2), pp. 38-46, March 2018



Shoot tip splitting for rapid micropropagation of Philippine taro [Colocasia esculenta (L.) Schott]

V. Z. Acedo1,2*, O. P. Damasco2, A. C. Laurena2, P. C. Sta Cruz2, L. O. Namuco2 and A. G. Lalusin2

1Philippine Root Crop Research and Training Center, Visayas State University, Baybay City, Leyte, Philippines.
2Crop Science Cluster, University of the Philippines at Los Banos, College, Laguna, Philippines.

*To whom correspondence should be addressed. E-mail: luzacedo@yahoo.com.

Received 25 February, 2018; Received in revised form 21 March, 2018; Accepted 24 March, 2018.

Abstract


Keywords:
Tissue culture, Shoot tip culture, Cultivar response.


This study was conducted to establish a micropropagation system for Philippine taro cultivars by shoot tip culture and explore the use of shoot tip splitting in increasing propagation rate. Four recommended cultivars (VG1, VG2, NG9 and NG10) were used. One centimeter shoot tip explants were aseptically prepared, cut longitudinally to produce half and quarter shoot tips, and cultured on modified Murashige and Skoog (MS) medium supplemented with 1-5 mg/L benzylaminopurine (BAP) or 0.5-1.0 mg/L indoleacetic acid (IAA) or their combination. The micropropagation protocol developed was simple and straight forward as the modified MS medium sufficed. Shoot tip splitting remarkably promoted shoot formation, with the quarter shoot tips producing the highest number of shoot-forming explants (80-100%) as compared to half shoot tips (50-60%) and whole shoot tips (20-40%) in all cultivars except NG10 where shoot-forming explants did not significantly vary with explant size. Rooting response of half and quarter shoot tip explants was either comparable to or higher than that of whole shoot tips but the time period to root formation was delayed in split shoot tips by 2-10 days relative to that of whole shoot tips. Because of more explants per shoot tip and the promotion of shoot and plantlet development, micropropagation ratio increased with the use of half and quarter shoot tips by 3 and 8 fold in VG1, 10 and 32 fold in VG2, 7 and 10 fold in NG9 and 4 and 7.6 fold in NG10, respectively. After 3-4 weeks, plantlets were potted out and following the ex vitro protocol, all plantlets survived with no visible morphological variation.

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