International Journal of Advance Agricultural Research
ISSN: 2053-1265
Vol. 7(4), pp. 98-107, June 2019
doi.org/10.33500/ijaar.2019.07.007



Effects of arbuscular mycorrhiza fungi on stimulation of nutrient content and induction of biochemical defense response in Xanthosoma sagittifolium plants against root rot disease caused by Pythium myriotylum

Ntengna Y. F.1, Tchameni N. S.1,5*, Fokom R.2,5, Sameza M. L.1, Minyaka E.1, Ngonkeu M. E. L.3,
Nana Wakam L.4, Etoa F. X.1 and Nwaga D.5*

1Laboratory of Biochemistry, Faculty of Science, University of Douala, P.O. Box 24 157 Douala, Cameroon.
2Institute of Fisheries and Aquatic Science (Yabassi), University of Douala, Douala, Cameroon.
3Department of Plant Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.
4Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.
5Soil Microbiology Laboratory, Biotechnology Centre, University of Yaoundé I, Yaoundé, Cameroon.

*To whom correspondence should be addressed. E-mail: tchameni1@yahoo.fr.

Received 29 March, 2019; Received in revised form 12 May, 2019; Accepted 16 May, 2019.

Abstract


Keywords:
AM fungi, Pythium myriotylum, Flavonones, Defense related enzymes, Disease tolerance.


This study was carried out to evaluate the effect of two selected arbuscular mycorrhizal (AM) fungi on cocoyam (Xanthosoma sagittifolium) plant growth, nutrition and bio protection against rot root. Cocoyam seedlings were inoculated with Acaulospora tuberculata and Gigaspora margarita in sterilize pot soil. Twelve weeks after planting, rhizosphere plant were infected with Pythium myriotylum and two weeks later, they were harvested for investigation of morphology, functioning and molecular changes related to plant defence response. The results showed that, the colonization of cocoyam plants with both AM fungal species significantly increase plant shoot, root dry matter, nutrients content and reduce disease incidence. Higher activities of defense related enzymes [phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO)], total phenol content and flavonone accumulation such as apigenin-pentosyl-hexoside, lutein-pentosyl-hexoside, caffeic acid and apigenin-dihexoside were obtained in mycorrhizal plants. This suggested the implication of these components in mycorrhizal cocoyam plants against P. myriotylum. AM fungi could be used as biological control agents to fight against Oomycete plant pathogens after assessments under field trials.

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