International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 4(7), pp. 109-116, November 2016
doi.org/10.33500/ijambr.2016.04.013



Rapid detection of extended spectrum β-lactamases production in Escherichia coli

Samir I. Abdelhady, Nadia M. ElSheshtawy* and Nesma A. Hamdi

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

*To whom correspondence should be addressed. E-mail: nadiamohamed@med.asu.edu.eg.

Received 16 September, 2016; Received in revised form 10 October, 2016; Accepted 13 October, 2016.

Abstract


Keywords:
Escherichia coli, Extended spectrum β-lactamases, Modified double disc synergy test, Nordmann-Dortet-Poirel test.


Multidrug resistance is a major threat causing a wide range of infections worldwide. The Enterobacteriaceae family is held responsible for many cases of antibiotic resistance, mainly by the means of extended spectrum β-lactamases (ESBLs) production. The modified double disc synergy test (MDDST) is a reliable method for ESBL detection with several drawbacks. The ESBL Nordmann-Dortet-Poirel (NDP) test can be used as a sensitive, specific and rapid alternative method for ESBL detection. This study was designed to evaluate the performance of NDP test for rapid detection of ESBL-producing Escherichia coli in comparison with MDDST. The research was carried out on 50 clinical E. coli and 25 fecal E. coli isolates from healthy controls. The isolates were tested for ESBL production by double disc diffusion test (DDT), MDDST and NDP; comparing the sensitivity and specificity of the three tests. Statistical Package for the Social Sciences (SPSS) version 22 was used for data analyses. P>0.05 was regarded as non-significant, P≤0.05 significant and P≤0.01 highly significant. It was observed that the sensitivity of NDP test was 100% while MDDST's sensitivity was 83.3%. The specificities of both NDP test and MDDST were 100%, respectively. MDDST yielded results after 3 days while NDP test gave results after 20-45 min. Therefore, NDP test was more rapid and more sensitive than MDDST as regards detection of ESBL producing E. coli. It can therefore be used for the detection of ESBL production instead of MDDST as it gave higher positive results than the MDDST and also bypassed the errors produced by unique inhibitor-resistant beta-lactamase.

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