International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 5(7), pp. 75-81, August, 2017



Prevalence, serotype and presence of invasion gene in Salmonella isolated from frog meat obtained from western region of Côte d’Ivoire

Adjehi Dadié1, Yatanan Casimir Blé1*, Kouadio-Ngbesso Nadège2, Agathe Fantodji3 and Koffi Marcelin Djè1

1Department of Food Science and Technology, Laboratory of Biotechnology and Food Microbiology, University Nangui Abrogoua 02 BP 801 Abidjan 02, Côte d’Ivoire.
2Oceanographic Research Center, BP V18, Abidjan, Côte d’Ivoire.
3Department of Animal Production, Laboratory of Cytology Animal, Université Nangui Abrogoua 02 BP 801 Abidjan 02, Côte d’Ivoire.

*To whom correspondence should be addressed. E-mail: yatanan12@hotmail.fr.

Received 20 December, 2016; Received in revised form 16 January, 2017; Accepted 19 January, 2017.

Abstract


Keywords:
Salmonella, Serotype, invA gene, Frog.

This study was carried out to investigate the prevalence of Salmonella, serogrouping and presence of invA gene (Salmonella Invasion Gene A) of Salmonella strains obtained from Hoplobatrachus occipitalis in Côte d’Ivoire. A total of 210 fresh and 384 smoked frogs from three local market were evaluated for the presence of Salmonella. Biochemical identification, serotyping test and detection of invA gene by polymerase chain reaction (PCR) were performed. The results obtained indicate that all 384 smoked frog was not contaminated by Salmonella sp. while, 11.4% of fresh frog were positive for Salmonella. There was no significant difference between the contamination rate according to sites (P>0.05). The serotyping results obtained show that isolated strains belong to four serotypes and the most dominant serotype was Salmonella lindenburg (55.5%). PCR reaction showed that 86.7% of the Salmonella possessed invA gene. The presence of Salmonella in edible frog indicates that there is a health risk for consumers and this requires monitoring in order to minimize the risk of contamination to members of the public.

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