International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 5(6), pp. 68-74, July, 2017



Molecular typing of Clostridium perfringens strains isolated from cooked beef, by toxin genes amplification

Kra Athanase KOUASSI1,2,3*, Adjéhi Thomas DADIE2, Kouadio Florent N’GUESSAN2, Kouadio YAO2,3,
Zinzendorf Yéssé NANGA3,4, Yao Guillaume LOUKOU3,4 and Koffi Marcellin DJE2

1Laboratory of Microbiology, Department of Food Microbiology, University Jean Lorougnon Guédé of Daloa, Côte d’Ivoire.
2Laboratory of Biotechnology and Food Microbiology, Department of Food Microbiology, University of Nangui Abrogoua, Abidjan, Côte d’Ivoire.
3National Laboratory of Public Health, Abidjan, Côte d’Ivoire.
4Department of Pharmaceutical and Biological Sciences, University of Felix Houphouet Boigny, Abidjan, Côte d’Ivoire.

*To whom correspondence should be addressed. E-mail: kraathanase@yahoo.fr.

Received 22 April, 2017; Received in revised form 12 May, 2017; Accepted 15 May, 2017.

Abstract


Keywords:
Cooked beef, Clostridium perfringens, Toxin genes, Multiplex PCR.

The aim of the study was to determine the presence of gene coding for alpha (cpa), beta (cpb), epsilon (etx), iota (iA) and enterotoxin (cpe) from Clostridium perfringens strains isolated from cooked beef sold in the streets. A total of 395 samples of cooked beef were collected from vendors at Abidjan Côte d’Ivoire and subjected to Clostridium perfringens isolation and identification by using biochemical tests and API 20A system. Then, the multiplex polymerase chain reaction (PCR) method was used for toxin typing for confirmed isolates. The results obtained show that the prevalence of C. perfringens in cooked beef sold in the streets varied from 0 to 14% according to the area of study. The mean value was 5.06% and spore count was 1.11 ufc/g. The 20 confirmed isolates belonged to two toxinotypes: type A (95%) and type B (5%). Two groups of C. perfringens type A were present in cooked beef sold in the streets. One group was cpe negative and represented 55% of the isolates. The second group harbored cpe gene in addition to cpa gene. This group was found at 40%.

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