International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 5(9), pp. 103-109, October, 2017



Antibiotic susceptibility and microbial analysis of Enterobacteriaceae from wastewater and sediments from abattoirs in Makurdi, Benue State, Nigeria

Omoregbe F. B.*, Ebar E. E. and Nevkaa D. N.

Department of Biological Sciences, University of Agriculture Makurdi, Benue State, Nigeria.

*To whom correspondence should be addressed. E-mail: omoregbeflorence@gmail.com.

Received 05 July, 2017; Received in revised form 20 September, 2017; Accepted 25 September, 2017.

Abstract


Keywords:
Wastewater, Sediment, Antibiotics, Resistance.

In Nigeria, the operation of many abattoirs is unregulated. Waste from these abattoirs therefore portend serious hazard to public health. To ascertain this, wastewater and sediment samples from four abattoirs in Makurdi, Benue State were investigated for antibiotic susceptibility and microbial analysis using the dilution pour plate method on Salmonella-shigella Agar (SSA) and MacConkey Agar for isolation of Enterobacteriaceae. While their antibiotic susceptibility pattern was studied using the method of National Committee for Clinical Laboratory Standards (NCCSLS). The result obtained show that mean values of bacterial count ranged from 2.00×106 – 1.37×108 cfu/ml for wastewater samples and 1.09×107 – 8.66×107 cfu/ml for sediment samples. The following genera of Enterobacteriaceae; Escherichia, Klebsiella, Shigella and Salmonella were isolated. Out of the 106 isolates obtained, 31(29.25%) were Escherichia spp., 28(26.42%) Salmonella spp., 25(23.58 %) Shigella spp., and 22(20.75%) Klebsiella spp. All isolated Salmonella spp. were completely resistant to ceftazidime, and ceftriazone while Escherichia coli and Klebsiella spp., were completely resistance to ertapenem, ceftazidime, and ceftriazone. Imipenem was the most potent antibiotic as all bacteria isolates were highly susceptible to it. The results obtained show that pathogenic species such as Salmonella and Shiegella were present in significant numbers in the abattoir wastewater and sediment with varying degree of resistance to antibiotic tested. Therefore, there is need for treating abattoir waste before discharge.

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