International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 7(7), pp. 106-116, August, 2019
doi.org/10.33500/ijambr.2019.07.013



Selection of Lactobacillus paracasei strains from grated carrots by-products for L-lactic acid production

Justine Godard*and Yann Demarigny

Univ Lyon, ISARA Lyon, Université Lyon 1, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), EA n°3733, ISARA, Agrapôle, 23 rue Jean Baldassini, F-69364, LYON, cedex 07, France.

*To whom correspondence should be addressed. E-mail: jgodard@isara.fr, ydemarigny@isara.fr.

Received 24 May, 2019; Received in revised form 19 June, 2019; Accepted 28 June, 2019.

Abstract


Keywords:
Vegetable fermentation, Carrot pulps, L-lactic acid production.

Beside its utilisation as food preservative, lactic acid can also be used to make biopolymers, or to enter in the composition of cleaning products. Nowadays, different ways are explored to optimize its production. The use of vegetable wastes could appear as a profitable option to reduce costs. In this work, 40 different native lactic acid bacteria isolated from fermented carrot pulps were analysed and compared for their ability to produce lactic acid. The selection relied on the use of two model media, the synthetic MRS medium and a sterilized carrot-like medium. This allowed for the isolation of two interesting species: Lactobacillus paracasei and Lactobacillus harbinensis, which produced high concentrations of L-lactic acid in MRS broth. However, the yield obtained with the carrot-like medium was less important and will have to be optimized in the future. L. paracasei proved to be more interesting than L. harbinensis. In particular, the strain A1L was able to metabolize starch. This ability could be a selective advantage in a complex vegetable matrix to overwhelm the other adventitious microorganisms.

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