International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 7(6), pp. 79-85, July, 2019
doi.org/10.33500/ijambr.2019.07.010



Exopolysaccharide production by Brevundimonas diminuta isolated from Marchica lagoon ecosystem in Morocco

Yigerta Djolo Dah Dossounon* and Mohammed Blaghen

Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, University Hassan II, Faculty of Sciences Ain-Chock, route El Jadida, B. P. 5366, Casablanca, Morocco.

*To whom correspondence should be addressed. E-mail: djolounivcasa@gmail.com.

Received 03 January, 2019; Received in revised form 21 May, 2019; Accepted 29 May, 2019.

Abstract


Keywords:
Emulsifying activities, Brevundimonas diminuta, Marchica lagoon.

Polysaccharides are the most diverse families of biopolymers. Brevundimonas diminuta, a member of the Class Bacilli, has the ability to synthesize and secrete polysaccharides. The strain isolated from Marchica lagoon in Morocco produced extracellular polysaccharides (EPS), mainly during its exponential growth phase but also to a lesser extent during the stationary phase. The optimum pH and temperature for growth and exopolysaccharides (EPSs) production were 8 and 37°C, respectively. The dry weight of the polysaccharides products and biomass was found to be 306.85 ± 4.45 mg/100 mL and 77.75 ± 2.47 mg/100 ml, respectively. B. diminuta did not produce EPS with galactose as the carbon source and the addition of peptone improved EPS production. There is no production of EPS in the absence of mineral salts. Screening for emulsifying activities showed emulsion for hexane and toluene.

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