International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 7(2), pp. 7-17, March, 2019
doi.org/10.33500/ijambr.2019.07.002



Diversity and antifungal activity of endophytes associated with Spiranthes sinensis (Orchidaceae, Magnoliophyta) in China

Dong Fang Pei1, Qiu Qiu Wu1, Huan Luo1, Narayan Chandra Paul2, Jian Xin Deng1* and Yi Zhou1*

1Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China.
2Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Korea.

*To whom correspondence should be addressed. E-mail: dongfangpei9@163.com, zhouyi@yangtzeu.edu.cn.

Received 16 February, 2019; Received in revised form 22 March, 2019; Accepted 25 March, 2019.

Abstract


Keywords:
Endophytic bacteria, Endophytic fungi, ITS, 16S rRNA.

Spiranthes sinensis (Pers) Ames is an important traditional medicinal plant under the Orchidaceae in China. This study investigated the diversity and antifungal activity of endophytes isolated from the leaves, stems, inflorescences and roots of S. sinensis collected in Chongqing, China. A total of 263 endophytes were obtained in this study. Endophytic fungi (57) and endophytic bacteria (48) were identified based on internal transcribed spacer rDNA regions (ITS) and 16S ribosomal RNA (16S rRNA) gene analyses, respectively. The results indicate that the endophytes were assigned to 25 genera of fungi and 11 genera of bacteria. The most frequently isolated endophytic fungi was Fusarium (20%) followed by Alternaria (8%) and Penicillium (8%). The dominant endophytic bacteria were Burkholderia (19%), Bacillus (18%) and Pseudomonas (17%). Antifungal activity of the endophytic strains (n=105) were evaluated against three agricultural plant pathogens (Fusarium oxysporum, Botrytis cinerea, Alternaria solani). Among them, 9 fungal and 8 bacterial isolates showed broad spectrum antifungal activity against at least one of the three pathogens. Especially, the endophytic bacteria of Bacillus velezensis and B. siamensis exhibited the best inhibitory activity against the three pathogens.

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