International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 7(4), pp. 39-45, May, 2019

Assessing the efficacy and mass production of fungal entomopathogens associated with Macrotermes bellicosus

Omoya, F. O.1 and Kelly, B. A.2*

1Department of Microbiology, Federal University of Technology, Akure, Nigeria.
2Biological Sciences Department, Wesley University, Ondo, Nigeria.

*To whom correspondence should be addressed. E-mail:,

Received 17 January, 2018; Received in revised form 02 April, 2019; Accepted 04 April, 2019.


Entomopathogen, Metarhizium anisopliae, Byproducts, Diseased.

Macrotermes bellicosus (Family: Termitidae, Order: Isoptera) adult workers were harvested from the farm of Federal University of Technology, Akure, Nigeria. They were allowed to acclimatized in the laboratory and were observed for the onset of disease symptoms. Fungi were isolated from diseased M. bellicosus using generalized and dodine based media. Isolated fungi were investigated for their lethality on the insects. The minimum concentration of the fungal entomopathogen (LD50) required to kill at least 50 percent of the test insects was determined. Mass production of the entomopathogens was evaluated on selected organic by-products. Analyses were carried out in triplicates and results were plotted on bar charts with the error bars indicating the standard deviation from the average values. The results obtained show that Metarhizium anisopliae was able to cause disease in worker termites used. The minimum lethal concentration (LD50) of the fungi required to induce pathogenicity was 5.0×105 spore forming units per milliliters. Mass production of the microorganism carried out by inoculating the entomopathogen onto organic by-products also showed the growth of M. anisopliae on maize shaft, rice water and brewer’s mash. M. anisopliae can be a suitable biocontrol agent for the control of M. bellicosus in Ondo state, Nigeria. It also shows that the organism has the potential to be mass produced using cheap organic by-products.

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