International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 8(1), pp. 1-14, March, 2020
doi.org/10.33500/ijambr.2020.08.001



Isolation, identification and evaluation of antioxidant, anti-inflammatory and antimitotic properties of bioactive pigment from Rhodococcus corynebacterioides SCG11

Chandrashekhar Naik*, Shivasharanappa Chandrappa Gadal, Rajeshwari T., Aparna R. Bhat, Sharanya B. and Chandini L.

Downstream Bioprocessing Laboratory, Department of Biotechnology, Sir M Visveavaraya Institute of Technology, Yelahanka, International Airport Road, Bangalore 562157.

*To whom correspondence should be addressed. E-mail: cknaikg@gmail.com.

Received 16 January, 2020; Received in revised form 20 February, 2020; Accepted 25 February, 2020.

Abstract


Keywords:
Bio-pigments, Rhodococcus corynebacterioides, Antioxidant, Anti-inflammatory, Antimitotic, GC-MS.

In an examination during the cause of this study, yellow-orange producing bacterium was isolated from pharmaceutical effluent to assess its antioxidant, anti-inflammatory and antimitotic properties. Morphological and molecular (16s rRNA) characterization delighted the confine as a Gram-positive bacilli Rhodococcus corynebacterioides and assigned as SCG11. The 16S rRNA sequence was submitted to NCBI Gen Bank with accession number: KU995335 and gene ID: 1015917315. The phylogenetic investigation with most extreme probability technique indicated 100% closeness with Rhodococcus spp. PBTS1. Thin layer chromatography was done for extracted pigment with various solvents system; acetonitrile and water (8:2, v/v) was observed to be the best solvent system and indicated distinct separations. The pigment was additionally purified through silica gel column chromatography and purified pigment was exposed to GC-MS examination. GC-MS results reviled the presence of ~29 compounds out of which hexadecanoic acid methyl ester (peak area % = 5.75), n-hexadecanoic acid (peak area % = 7.59) have antioxidant properties, n-hexadecanoic acid (peak area % = 7.59) and ergotaman-3’,6’,18-trione,9,10-dihydro-12’-hydro-2’-methyl’5-(phenyl methyl)-,(5’α,10α)- (peak area % = 3.19), ornithine (peak area % = 6.84) have anti-inflammatory properties and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- (peak area % = 3.78) has antimitotic properties.

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