International Journal of Modern Biological Research
ISSN: 2053-180X
Vol. 4(2), pp. 5-15, February 2016



Role of human umbilical cord blood derived- mononuclear cells-versus CD34 cells on chronic renal failure in adult male albino rats

Mona Hassan Mohammed Ali1* and Hala Abdel Dayem Mohamed2

1Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
2Department of Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

*To whom correspondence should be addressed. E-mail: hararh2002@yahoo.com.

Received 12 January, 2016; Received in revised form 02 February, 2016; Accepted 08 February, 2016.

Abstract


Keywords:
Renal failure, Mononuclear cells, HUCBSCs, CD34 cells, Nephrectomy model.


Chronic renal failure (CRF) has become a public health problem. The therapeutic approaches have not been successful in limiting disease progression. The therapeutic potential of adult stem cells for the treatment of chronic diseases is becoming increasingly evident over the last few years as the current therapeutic opportunities to slow the progression of the disease are limited. We attempted to assess the therapeutic effectiveness of mononuclear cells (MNCs) versus CD34+ cells derived from human umbilical cord blood stem cells (HUCBSCs) in chronic renal failure rat model. The 5/6 nephrectomy model was used in rats to induce chronic renal failure. Forty inbred strain adult albino male were allocated randomly to 4 equal groups: A control group G1, CRF groups (CRF with saline infusion group) G2, CRF/MNCs group G3 and CRF/HUCB CD34+ cells group G4. Systolic blood pressure, renal functioning tests, histological examination and immunohistochemistry for laminin expression were assessed. Treatment with MNCs and CD34+ cells significantly decreased serum creatinine and urea as well as systolic blood pressure with concomitant decrease in the degree of fibrosis indicated histologically by decreased renal laminin expression. There was a significant difference in these parameters between MNCs and CD34+ stem cells treated groups. There was positive reaction for antibody anti-rat laminin in 80% of animals in MNCs treated rats versus 60% in CD34+ cells treated rats. HUCB derived CD34+ and MNCs accelerate regeneration of renal tubular epithelial cells and lead to reduction of progressive renal injury in induced renal failure rats.

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