International Journal of Modern Biological Research
ISSN: 2053-180X
Vol. 5(3), pp. 42-55, August 2017



Synthesis of silver nanoparticles of mesocarp of Lagenaria siceraria fruit by using oleylamine and evaluation of its antimicrobial potential against clinically used pathogens

Parsa Dar1*, Amara Dar2, Azam Mehmood Dar3 and Usama Waqas4

1Department of Chemistry, Quaid-i-Azam University Islamabad, 45320, Pakistan.
2Center for Undergraduate Studies, University of the Punjab, Lahore- 54590, Pakistan.
3Institute of Chemical Engineering and Technology, University of the Punjab, Lahore-54590, Pakistan.
4Department of Chemistry, Govt. College University, Lahore-54000, Pakistan.

*To whom correspondence should be addressed. E-mail: parsadar@yahoo.com.

Received 08 July, 2017; Received in revised form 25 July, 2017; Accepted 28 July, 2017.

Abstract


Keywords:
Silver nanoparticles, Lagenaria siceraria, Green synthesis, Antibacterial, Antifungal.


The field of nanotechnology is mushrooming and influencing human life very rapidly. Using plants for the synthesis of nanoparticles, is evolving as a new branch of nanotechnology. In plants, wide-ranging biomolecules act as reducing and capping agents, thereby increasing the reduction rate and stabilization of nanoparticles. The present study dealt with green synthesis of silver nanoparticles (AgNPs) using methanolic extract of Lagenaria siceraria fruit mesocarp and its various fractions. The formation and characterization of AgNPs is established by UV-visible spectroscopy, fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The antimicrobial assays were conducted against bacterial strains including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and fungal strain including Candida albicans by agar well diffusion method.

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