International Journal of Modern Biological Research
ISSN: 2053-180X
Vol. 5(1), pp. 1-7, February 2017



Ethanolic extracts of different fruit trees and their activity against Strongyloides venezuelensis

Letícia Aparecida Duart Bastos1, Marlene Tiduko Ueta1, Vera Lúcia Garcia2, Rosimeire Nunes de Oliveira1,
Mara Cristina Pinto3, Tiago Manuel Fernandes Mendes1 and Silmara Marques Allegretti1*

1Biology Institute, Animal Biology Department, Campinas State University (UNICAMP), SP, Brazil.
2Multidisciplinary Center of Chemical Biological and Agricultural Research (CPQBA), Campinas State University (UNICAMP), Paulínia, SP, Brazil.
3São Paulo State University (UNESP), Araraquara, SP, Brazil.

*To whom correspondence should be addressed. E-mail: sallegre@unicamp.br.

Received 5 December, 2016; Received in revised form 2 January, 2017; Accepted 5 January, 2017.

Abstract


Keywords:
Rodent parasite, Diagnostic test, Fruit trees, Anti-strongyloides activity, Anthelmintic effect.


Strongyloides venezuelensis and Strongyloides ratti are both rodents’ parasites that are important models both in an immunologic and biologic perspective, for the development of new drugs and diagnostic tests. This study was aimed at studying the anthelminthic in vitro effect of ethanolic extracts, obtained from several species of Brazilian fruit trees, against S. venezuelensis parasitic females in an attempt to search for new therapeutic alternatives. Plant leaves were collected in the Multidisciplinary Center of Chemical, Biological and Agricultural Research (CPQBA) at Campinas State University (UNICAMP), in Paulínia, Brazil. Ethanolic extracts were obtained by mixing dried powdered plant leaves (10 g) with 150 mL of ethanol for 10 min/16.000 rpm in a mechanical disperser (Ultra Turrax T50, IKA Works Inc., Wilmington, NC, USA), followed by filtration. The residue was re-extracted with 100 mL of ethanol. The extracts were pooled and evaporated under vacuum until dry, resulting in the final dried ethanolic extracts. Inhibitory concentration was determined using Origin 7 program. Statistical analysis was performed using SAS program. Significant differences between groups were calculated using one-way Analysis of Variance (ANOVA) (p<0,0001). The correlation between time, motility, oviposition, and mortality with the extract concentration was accessed using Duncan’s Multiple Range Test. Spondias mombin ethanolic extract and aqueous fraction showed the most promising results, with anti-Strongyloides activity and 100% mortality rate after 72 h, for all tested concentrations. Overall, most of the extracts showed a satisfactory anthelmintic effect for at least one of the tested concentrations.

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