Journal of Medicinal Plant and Herbal Therapy Research
ISSN: 2053-1826
Vol. 6(2), pp. 19-30, July 2018



Evaluation of wound healing activity of Achidium ohioense (Schimp. ex Mull) oil on albino rats

Akinpelu B. A.1*, Odukoya S. O. A.2, Akanni A. A.1, Adelodun S. T.2 and Oyedapo O. O.1

1Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
2Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

*To whom correspondence should be addressed. E-mail: baakinpelu@gmail.com.

Received 16 February, 2018; Received in revised form 16 July, 2018; Accepted 19 July, 2018.

Abstract


Keywords:
Wound healing, Archidium ohioense, Antioxidant, Enzymes, Connective tissue markers, Free radicals.

The study evaluated Archidium ohioense oil potential in wound management with three different wound models in rats. The rats were subjected to incision, excision and dead space models measuring 6 cm, 500 mm2 and 1 cm, respectively. A. ohioense oil (0.5 and 1.0 mg/kg bwt) was administered topically, once daily for 10 days (incision and dead space models) or for 21 days or more (excision wound model) in rats. In vivo studies on wound breaking strength, rate of contraction, period of epithelization (excision model), granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants [catalase (CAT) superoxide dismutase (SOD) and reduced glutathione (GSH)], connective tissue markers (hydroxyproline, hexosamine and hexuronic acid) and deep tissue histology (dead space model) were investigated. The results obtained reveal that A. ohioense oil enhanced wound breaking strength and contraction rate, tissue collagen formation and early epithelization period. There was decrease in the levels of free radical, enhanced antioxidant and connective tissue markers alongside histological substantiation of formation of more collagen in skin and deeper connective tissue. The findings of this study indicate that A. ohioense oil possessed wound healing activity when applied topically.

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