International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 8(4), pp. 40-50, September, 2020
doi.org/10.33500/ijambr.2020.08.005

Establishment of cell suspension culture with anti-inflammatory activity and the effect of salicylic acid on the production of callus from Cnidoscolus chayamansa Mc Vaugh

Mariana Zuleima Pérez-González, María del Carmen Juárez-Vázquez and María Adelina Jiménez-Arellanes*.

Unidad de Investigación Médica en Farmacología, Hospital de Especialidades, Instituto Mexicano del Seguro Social (IMSS), Centro Médico Nacional Siglo XXI. Av. Cuauhtémoc 330, Col. Doctores, C.P. 06720, CDMX, Mexico.

*To whom correspondence should be addressed. E-mail: adelinajim08@prodigy.net.mx.

Received 15 July, 2020; Received in revised form 08 August, 2020; Accepted 24 August, 2020.

Keywords:
Cnidoscolus chayamansa, Suspension cell culture, Scopoletin, Anti-inflammatory activity, Lupeol acetate.

Cnidoscolus chayamansa Mc Vaugh (Euphorbiaceae), known as Chaya, Mexican spinach or Mayan spinach, is of interest for its high nutraceutical value and broad medicinal uses. Previously, it has been described that C. chayamansa has several biological activities (antiprotozoal, antimycobacterial, gastroprotective, cardioprotective, antioxidant, hypoglycemic, anti-inflammatory and hepatoprotective) and from active extracts moretenol, moretenyl acetate, β-amirenone, β-amyrin acetate, moretenone, lupeol acetate, scopoletin, 3,7-dimethyl ether of kaempferol and 5-hydroxy-7-3', 4'-trimethoxyflavanone have been isolated. In this work, the biotechnology procedure to obtain callus and cell suspension using a combination of auxin [NAA (2.5 mg/L)], cytokinin [BAP (5 mg/L)] and sucrose (30 g/L) as a carbon source is described. In addition, high-resolution liquid chromatography (HPLC) analysis of the organic extracts from cell line showed that it contains scopoletin (3.68 mg/g dry extract); while by thin layer chromatography (TLC) analysis moretenol acetate (minor compound), β-sitosterol, p-cumaric acid, cinnamic acid and kaempferol were identified. The organic extract from cells was inactive in the topical inflammation (TPA) and systemic (carrageenan) models. Finally, elicitation with salicylic acid was performed, showing that callus formation was in a shorter time (28 days) than the crop with plant growth regulators (50 days) with a similar percentage of callogenesis of 77.77 and 75%, respectively. This research can be a pioneer for the production of metabolites of biological interest from this medicinal plant.

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